Phospho-POLR2A-S2 pAb
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P24928 |
| Gene Id | 5430 |
| Gene Symbols | POLR2A |
| Protein Name / Synonyms | POLR2A, POLR2, POLRA, RPB1, RPBh1, RPO2, RPOL2, RpIILS, hRPB220, hsRPB1, RNA polymerase II subunit A |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
Dilutions
WB 1:1000 - 1:2000
IF 1:50 - 1:100
IP 1:50 - 1:100
IF 1:50 - 1:100
IP 1:50 - 1:100
Format
Calculated MW
63kDa/217kDa
Observed MW
260kDa
Immunogen
A phospho specific peptide corresponding to residues surrounding S2 of human POLR2A
Modification
Phospho S2
Storage
-20°C
Images
Western blot analysis of extracts of 293T cells, using Phospho-POLR2A-S2 antibody (144-61509) at 1:1000 dilution. 293T cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37? for 1 hour or treated by Doxorubicin (0.5uM) for 24 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunofluorescence analysis of HeLa cells using Phospho-POLR2A-S2 antibody (144-61509) at dilution of 1:100. HeLa cells were treated by nocodazole (50 ng/ml) at 37? for 20 hours (left). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.