POR Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P16435 |
| Gene Id | 5447 |
| Gene Symbols | POR |
| Protein Name / Synonyms | POR, CPR, CYPOR, P450R, cytochrome p450 oxidoreductase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
76kDa
Observed MW
77kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human POR (NP_000932.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using POR antibody (144-08142) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.![Western blot - [KO Validated] POR Polyclonal Antibody (144-08142)](https://cdn.sanity.io/images/g51potcz/production/4e5e7c1706fb1f654dbe5be14bf7b9ba2e9988ef-640x350.jpg)
Western blot analysis of extracts from normal (control) and POR knockout (KO) HeLa cells, using POR antibody (144-08142) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.![Western blot - [KO Validated] POR Polyclonal Antibody (144-08142)](https://cdn.sanity.io/images/g51potcz/production/7ce2c79094907d8cbb7bff402411ccb8d682055a-350x350.jpg)
Immunohistochemistry of paraffin-embedded human esophagus using POR antibody (144-08142) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] POR Polyclonal Antibody (144-08142)](https://www.raybiotech.com/files/images/Antibodies/144/144-08142/3.jpg)
Expiration:
12 months from the date of shipment when stored properly.