POU5F1 Polyclonal Antibody
As low as $166.00
In stock
Only %1 left

Product Description


Size 50 ul, 100 ul, 200 ul
Species Human, Mouse, Rat
Host Species Rabbit
Clonality Polyclonal
Isotype Igg
Buffers 50% glycerol, PBS with 0.02% sodium azide, pH7.3.
Protein Name / Synonyms POU5F1, OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, POU class 5 homeobox 1
Accession Number Q01860
Gene Symbols POU5F1
Gene Id 5460
Purity Affinity purification


This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.


WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200


Calculated MW
Observed MW


A synthetic peptide of human POU5F1






Western blot analysis of extracts of various cell lines, using POU5F1 antibody (144-07920) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - POU5F1 Polyclonal Antibody (144-07920)

Immunohistochemistry of paraffin-embedded human brain cancer using POU5F1 antibody (144-07920) at dilution of 1:100 (40x lens).
Immunohistochemistry - POU5F1 Polyclonal Antibody (144-07920)

Immunofluorescence analysis of U2OS cells using POU5F1 antibody (144-07920) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - POU5F1 Polyclonal Antibody (144-07920)

12 months from the date of shipment when stored properly.
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