This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
A synthetic peptide of human POU5F1
Western blot analysis of extracts of various cell lines, using POU5F1 antibody (144-07920) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human brain cancer using POU5F1 antibody (144-07920) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using POU5F1 antibody (144-07920) at dilution of 1:100. Blue: DAPI for nuclear staining.
12 months from the date of shipment when stored properly.