PPOX Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P50336 |
| Gene Id | 5498 |
| Gene Symbols | PPOX |
| Protein Name / Synonyms | PPOX, PPO, V290M, VP, protoporphyrinogen oxidase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes the penultimate enzyme of heme biosynthesis, which catalyzes the 6-electron oxidation of protoporphyrinogen IX to form protoporphyrin IX. Mutations in this gene cause variegate porphyria, an autosomal dominant disorder of heme metabolism resulting from a deficiency in protoporphyrinogen oxidase, an enzyme located on the inner mitochondrial membrane. Alternatively spliced transcript variants encoding the same protein have been identified.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:100
Format
Calculated MW
50kDa
Observed MW
51kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PPOX (NP_001116236.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PPOX Antibody (144-06397) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human colon carcinoma using PPOX antibody (144-06397) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver injury using PPOX antibody (144-06397) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using PPOX antibody (144-06397) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using PPOX antibody (144-06397) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using PPOX antibody (144-06397). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.