PPP2R2A Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P63151 |
| Gene Id | 5520 |
| Gene Symbols | PPP2R2A |
| Protein Name / Synonyms | PPP2R2A, B55A, B55ALPHA, PR52A, PR55A, PR55alpha, protein phosphatase 2 regulatory subunit Balpha |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an alpha isoform of the regulatory subunit B55 subfamily. Alternatively spliced transcript variants have been described.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
51kDa/53kDa
Observed MW
52kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-447 of human PPP2R2A (NP_002708.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PPP2R2A antibody (144-02185) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat kidney using PPP2R2A antibody (144-02185) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using PPP2R2A antibody (144-02185) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using PPP2R2A antibody (144-02185) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.