PPP4C Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P60510 |
| Gene Id | 5531 |
| Gene Symbols | PPP4C |
| Protein Name / Synonyms | PPP4C, PP-X, PP4, PP4C, PPH3, PPP4, PPX, protein phosphatase 4 catalytic subunit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IP 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:100
IP 1:50 - 1:200
Format
Calculated MW
35kDa
Observed MW
36kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-307 of human PPP4C (NP_002711.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PPP4C antibody (144-02109) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat lung using PPP4C antibody (144-02109) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using PPP4C antibody (144-02109) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using PPP4C antibody (144-02109) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using PPP4C antibody (144-02109) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using PPP4C antibody (144-02109) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using PPP4C antibody (144-02109). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug PPP4C antibody (144-02109). Western blot was performed from the immunoprecipitate using PPP4C antibody (144-02109) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.