PRDX4 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q13162 |
| Gene Id | 10549 |
| Gene Symbols | PRDX4 |
| Protein Name / Synonyms | PRDX4, AOE37-2, AOE372, HEL-S-97n, PRX-4, peroxiredoxin-4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappaB.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
30kDa
Observed MW
28kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 38-271 of human PRDX4 (NP_006397.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PRDX4 antibody (144-01486) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.![Western blot - [KO Validated] PRDX4 Polyclonal Antibody (144-01486)](https://cdn.sanity.io/images/g51potcz/production/7e02f66da75fe95db50fc413bb594796032b546a-354x350.jpg)
Western blot analysis of extracts from normal (control) and PRDX4 knockout (KO) 293T cells, using PRDX4 antibody (144-01486) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.![Western blot - [KO Validated] PRDX4 Polyclonal Antibody (144-01486)](https://www.raybiotech.com/files/images/Antibodies/144/144-01486/2.jpg)
Expiration:
12 months from the date of shipment when stored properly.