PRMT5 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O14744 |
| Gene Id | 10419 |
| Gene Symbols | PRMT5 |
| Protein Name / Synonyms | PRMT5, HRMT1L5, IBP72, JBP1, SKB1, SKB1Hs, protein arginine methyltransferase 5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes an enzyme that belongs to the methyltransferase family. The encoded protein catalyzes the transfer of methyl groups to the amino acid arginine, in target proteins that include histones, transcriptional elongation factors and the tumor suppressor p53. This gene plays a role in several cellular processes, including transcriptional regulation, and the assembly of small nuclear ribonucleoproteins. A pseudogene of this gene has been defined on chromosome 4. Alternative splicing results in multiple transcript variants encoding different isoforms.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:200
IHC 1:50 - 1:100
IF 1:50 - 1:200
Format
Calculated MW
53kDa/66kDa/67kDa/71kDa/72kDa
Observed MW
70kDa
Immunogen
A synthetic peptide of human PRMT5
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PRMT5 antibody (144-01520) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Immunohistochemistry of paraffin-embedded rat brain using PRMT5 antibody (144-01520) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using PRMT5 antibody (144-01520) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using PRMT5 antibody (144-01520) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using PRMT5 antibody (144-01520). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.