Synonyms
IOTA antibody, MGC22756 antibody, MGC2333 antibody, MGC23846 antibody, PROS27 antibody, p27K antibody, PSMA6 antibody, 27 kDa prosomal protein antibody, multicatalytic endopeptidase complex iota chain antibody, macropain iota chain antibody, prosomal P27K protein antibody, proteasome subunit alpha type-6 antibody, PROS-27 antibody, macropain subunit iota antibody, proteasome iota chain antibody, proteasome subunit iota antibody, "proteasome (prosome, macropain) subunit, alpha type, 6 antibody"
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
27 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 234 (P60900) of Proteasome 20S alpha 6
Applications
Applications Tested
ICC/IF,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Sample (50 ug of whole cell lysate)
A: Mouse brain
12% SDS PAGE
107-10509 diluted at 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 20S Proteasome alpha 6(107-10509) antibody at 1:200 dilution.

Sample(30 ug whole cell lysate)
A:Hep G2
7.5% SDS PAGE
107-10509 diluted at 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.