PRPF8 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q6P2Q9 |
| Gene Id | 10594 |
| Gene Symbols | PRPF8 |
| Protein Name / Synonyms | PRPF8, HPRP8, PRP8, PRPC8, RP13, SNRNP220, pre-mRNA processing factor 8 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa.
Dilutions
WB 1:500 - 1:1000
IHC 1:50 - 1:100
IF 1:50 - 1:100
IP 1:50 - 1:100
IHC 1:50 - 1:100
IF 1:50 - 1:100
IP 1:50 - 1:100
Format
Calculated MW
273kDa
Observed MW
274kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PRPF8 (NP_006436.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PRPF8 antibody (144-06053) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human esophagus using PRPF8 antibody (144-06053) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using PRPF8 antibody (144-06053) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using PRPF8 antibody (144-06053) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U-251MG cells using PRPF8 antibody (144-06053) at dilution of 1:100 (20x lens). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.