PTK2 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q05397 |
| Gene Id | 5747 |
| Gene Symbols | PTK2 |
| Protein Name / Synonyms | PTK2, FADK, FAK, FAK1, FRNK, PPP1R71, p125FAK, pp125FAK, focal adhesion kinase 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Research Area | Angiogenesis, Cardiovascular Disease, Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
Format
Calculated MW
39kDa/48kDa/63kDa/99kDa/114kDa/119kDa/120kDa
Observed MW
119kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 700-800 of human PTK2 (NP_722560.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PTK2 antibody (144-60065) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human colon using PTK2 antibody (144-60065) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using PTK2 antibody (144-60065) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using PTK2 antibody (144-60065) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC12 cells using PTK2 antibody (144-60065) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of RAW264.7 cells using PTK2 antibody (144-60065) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug PTK2 antibody (144-60065). Western blot was performed from the immunoprecipitate using PTK2 antibody (144-60065) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.