This gene encodes a member of the receptor protein tyrosine kinase family of proteins that transduce extracellular signals across the cell membrane. The encoded protein lacks detectable catalytic tyrosine kinase activity, is involved in the Wnt signaling pathway and plays a role in multiple cellular processes including polarity and adhesion. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
WB 1:500 - 1:2000
IHC 1:50 - 1:100
Recombinant fusion protein containing a sequence corresponding to amino acids 811-1070 of human PTK7 (NP_002812.2).
Western blot analysis of extracts of various cell lines, using PTK7 antibody (144-09839) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Immunohistochemistry of paraffin-embedded mouse brain using PTK7 antibody (144-09839) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using PTK7 antibody (144-09839) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using PTK7 antibody (144-09839) at dilution of 1:100 (40x lens).
12 months from the date of shipment when stored properly.