PUF60 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9UHX1 |
| Gene Id | 22827 |
| Gene Symbols | PUF60 |
| Protein Name / Synonyms | PUF60, FIR, RoBPI, SIAHBP1, VRJS, poly(U) binding splicing factor 60 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IP 1:20 - 1:50
IHC 1:50 - 1:200
IF 1:50 - 1:100
IP 1:20 - 1:50
Format
Calculated MW
54kDa/55kDa/57kDa/58kDa/59kDa
Observed MW
65kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 243-542 of human PUF60 (NP_055096.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PUF60 antibody (144-06709) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using PUF60 antibody (144-06709) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using PUF60 antibody (144-06709). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug PUF60 antibody (144-06709). Western blot was performed from the immunoprecipitate using PUF60 antibody (144-06709) at a dilition of 1:500.
Expiration:
12 months from the date of shipment when stored properly.