RAB5A Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P20339 |
| Gene Id | 5868 |
| Gene Symbols | RAB5A |
| Protein Name / Synonyms | RAB5A, RAB5, ras-related protein Rab-5A |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Research Area | Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Dilutions
WB 1:500 - 1:2000
IF 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
22kDa/23kDa
Observed MW
22kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-215 of human RAB5A (NP_004153.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using RAB5A antibody (144-01180) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.![Western blot - [KO Validated] RAB5A Polyclonal Antibody (144-01180)](https://cdn.sanity.io/images/g51potcz/production/e3e880e4b808028f7d775779f7f35ad5f1f9f726-263x350.jpg)
Western blot analysis of extracts from normal (control) and RAB5A knockout (KO) HeLa cells, using RAB5A antibody (144-01180) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.![Western blot - [KO Validated] RAB5A Polyclonal Antibody (144-01180)](https://cdn.sanity.io/images/g51potcz/production/eeb151aa283a65a0154218fdc8103d442bc65262-367x550.jpg)
Immunofluorescence analysis of HeLa cells using RAB5A antibody (144-01180). Blue: DAPI for nuclear staining.![Immunofluorescence - [KO Validated] RAB5A Polyclonal Antibody (144-01180)](https://www.raybiotech.com/files/images/Antibodies/144/144-01180/3.jpg)
Expiration:
12 months from the date of shipment when stored properly.