[KO Validated] RHOC Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Rat |
| Host Species | Rabbit |
| Accession Number | P08134 |
| Gene Id | 389 |
| Gene Symbols | RHOC |
| Protein Name / Synonyms | RHOC, ARH9, ARHC, H9, RHOH9, ras homolog family member C |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
22kDa
Observed MW
22kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human RHOC (NP_001036144.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using RHOC antibody (144-01062).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.![Western blot - [KO Validated] RHOC Polyclonal Antibody (144-01062)](https://cdn.sanity.io/images/g51potcz/production/0f9d40b49cba8ba401284a11928489e3b8d6c2af-462x350.jpg)
Western blot analysis of extracts from normal (control) and RHOC knockout (KO) 293T cells, using RHOC antibody (144-01062) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 300s.![Western blot - [KO Validated] RHOC Polyclonal Antibody (144-01062)](https://cdn.sanity.io/images/g51potcz/production/e3234a507296448d4be02cc1a48da597f93652ef-366x550.jpg)
Immunofluorescence analysis of HeLa cells using RHOC antibody (144-01062). Blue: DAPI for nuclear staining.![Immunofluorescence - [KO Validated] RHOC Polyclonal Antibody (144-01062)](https://www.raybiotech.com/files/images/Antibodies/144/144-01062/3.jpg)
Expiration:
12 months from the date of shipment when stored properly.