Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P04843
Gene Id	6184
Gene Symbols	RPN1
Protein Name / Synonyms	RPN1, OST1, RBPH1, ribophorin I
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Shipping Type	Blue ice
Storage	-20°C

Background

This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

68kDa

Observed MW

69kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-280 of human RPN1 (NP_002941.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using RPN1 antibody (144-600827) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - RPN1 antibody (144-600827)

Immunohistochemistry of paraffin-embedded human colon using RPN1 antibody (144-600827) at dilution of 1:100 (40x lens).
Immunohistochemistry - RPN1 antibody (144-600827)

Immunohistochemistry of paraffin-embedded human gastric cancer using RPN1 antibody (144-600827) at dilution of 1:100 (40x lens).
Immunohistochemistry - RPN1 antibody (144-600827)

Immunohistochemistry of paraffin-embedded rat kidney using RPN1 antibody (144-600827) at dilution of 1:100 (40x lens).
Immunohistochemistry - RPN1 antibody (144-600827)

Immunohistochemistry of paraffin-embedded human tonsil using RPN1 antibody (144-600827) at dilution of 1:100 (40x lens).
Immunohistochemistry - RPN1 antibody (144-600827)

Immunohistochemistry of paraffin-embedded human liver cancer using RPN1 antibody (144-600827) at dilution of 1:100 (40x lens).
Immunohistochemistry - RPN1 antibody (144-600827)

Immunohistochemistry of paraffin-embedded human colon carcinoma using RPN1 antibody (144-600827) at dilution of 1:100 (40x lens).
Immunohistochemistry - RPN1 antibody (144-600827)

Immunohistochemistry of paraffin-embedded mouse testis using RPN1 antibody (144-600827) at dilution of 1:100 (20x lens).
Immunohistochemistry - RPN1 antibody (144-600827)

Immunohistochemistry of paraffin-embedded mouse spinal cord using RPN1 antibody (144-600827) at dilution of 1:100 (40x lens).
Immunohistochemistry - RPN1 antibody (144-600827)

Immunohistochemistry of paraffin-embedded mouse brain using RPN1 antibody (144-600827) at dilution of 1:100 (40x lens).
Immunohistochemistry - RPN1 antibody (144-600827)

Immunohistochemistry of paraffin-embedded mouse kidney using RPN1 antibody (144-600827) at dilution of 1:100 (40x lens).
Immunohistochemistry - RPN1 antibody (144-600827)

Expiration:

12 months from the date of shipment when stored properly.