WB 1:200 - 1:500
Recombinant fusion protein containing a sequence corresponding to amino acids 1-199 of human RPP25 (NP_060263.2).
Western blot analysis of extracts of various cell lines, using RPP25 antibody (144-09973) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
12 months from the date of shipment when stored properly.