WB 1:500 - 1:2000
IHC 1:100 - 1:200
Recombinant fusion protein containing a sequence corresponding to amino acids 1-268 of human RPP30 (NP_006404.1).
Western blot analysis of extracts of various cell lines, using RPP30 antibody (144-08479) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human lung cancer using RPP30 antibody (144-08479) at dilution of 1:100 (40x lens).
12 months from the date of shipment when stored properly.