RPS3A Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P61247 |
| Gene Id | 6189 |
| Gene Symbols | RPS3A |
| Protein Name / Synonyms | RPS3A, FTE1, MFTL, S3A, ribosomal protein S3A |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S3AE family of ribosomal proteins. It is located in the cytoplasm. Disruption of the gene encoding rat ribosomal protein S3a, also named v-fos transformation effector protein, in v-fos-transformed rat cells results in reversion of the transformed phenotype. This gene is co-transcribed with the U73A and U73B small nucleolar RNA genes, which are located in its fourth and third introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
Format
Calculated MW
29kDa
Observed MW
35kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human RPS3A (NP_000997.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using RPS3A antibody (144-05885) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using RPS3A Antibody (144-05885) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using RPS3A Antibody (144-05885) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using RPS3A Antibody (144-05885) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using RPS3A antibody (144-05885). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of MCF7 cells using 3ug RPS3A antibody (144-05885). Western blot was performed from the immunoprecipitate using RPS3A antibody (144-05885) at a dilition of 1:500.
Expiration:
12 months from the date of shipment when stored properly.