S100A10 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P60903 |
| Gene Id | 6281 |
| Gene Symbols | S100A10 |
| Protein Name / Synonyms | S100A10, 42C, ANX2L, ANX2LG, CAL1L, CLP11, Ca[1], GP11, P11, p10, protein S100-A10 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in exocytosis and endocytosis.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
11kDa
Observed MW
11kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-97 of human S100A10 (NP_002957.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using S100A10 antibody (144-60742) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat ovary using S100A10 antibody (144-60742) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using S100A10 antibody (144-60742) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using S100A10 antibody (144-60742) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using S100A10 antibody (144-60742) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human mammary cancer using S100A10 antibody (144-60742) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse leydig cells using S100A10 antibody (144-60742) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.