S100A4 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P26447 |
| Gene Id | 6275 |
| Gene Symbols | S100A4 |
| Protein Name / Synonyms | S100A4, 18A2, 42A, CAPL, FSP1, MTS1, P9KA, PEL98, protein S100-A4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
11kDa
Observed MW
12kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-101 of human S100A4 (NP_002952.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using S100A4 antibody (144-01631) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.![Western blot - [KO Validated] S100A4 Polyclonal Antibody (144-01631)](https://cdn.sanity.io/images/g51potcz/production/e4e48a95dc34ca8ff21f045245a3b7034fa650a1-230x350.jpg)
Western blot analysis of extracts from normal (control) and S100A4 knockout (KO) HeLa cells, using S100A4 antibody (144-01631) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 180s.![Western blot - [KO Validated] S100A4 Polyclonal Antibody (144-01631)](https://cdn.sanity.io/images/g51potcz/production/cce98d8501b19965716ba91a92f4dd61fa701d22-350x350.jpg)
Immunohistochemistry of paraffin-embedded rat brain using S100A4 antibody (144-01631) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] S100A4 Polyclonal Antibody (144-01631)](https://cdn.sanity.io/images/g51potcz/production/457ddc179a68dc4164e40c9003de6e89b60c318a-350x350.jpg)
Immunohistochemistry of paraffin-embedded human mammary gland using S100A4 antibody (144-01631) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] S100A4 Polyclonal Antibody (144-01631)](https://cdn.sanity.io/images/g51potcz/production/ba794c4a8f94234d86b6b7582b047d5b51d609f6-350x350.jpg)
Immunohistochemistry of paraffin-embedded human gastric cancer using S100A4 antibody (144-01631) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] S100A4 Polyclonal Antibody (144-01631)](https://cdn.sanity.io/images/g51potcz/production/76c99ba49d8caa03db37b54d2ec79c95c9b7483a-350x350.jpg)
Immunohistochemistry of paraffin-embedded mouse lung using S100A4 antibody (144-01631) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] S100A4 Polyclonal Antibody (144-01631)](https://cdn.sanity.io/images/g51potcz/production/4075fbd15acf72574890e2fe737f4ac09723b7f2-350x350.jpg)
Immunohistochemistry of paraffin-embedded human tonsil using S100A4 antibody (144-01631) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] S100A4 Polyclonal Antibody (144-01631)](https://cdn.sanity.io/images/g51potcz/production/5093e04b13103ba14364ab2418ea12f8acc4f9bb-440x550.jpg)
Immunofluorescence analysis of HeLa cells using S100A4 antibody (144-01631) at dilution of 1:100. Blue: DAPI for nuclear staining.![Immunofluorescence - [KO Validated] S100A4 Polyclonal Antibody (144-01631)](https://cdn.sanity.io/images/g51potcz/production/86ac4e217f97e4066b16a350474fadcdab2181ce-440x550.jpg)
Immunofluorescence analysis of PC12 cells using S100A4 antibody (144-01631) at dilution of 1:100. Blue: DAPI for nuclear staining.![Immunofluorescence - [KO Validated] S100A4 Polyclonal Antibody (144-01631)](https://cdn.sanity.io/images/g51potcz/production/67aebb7eb32efd480e6c66ccc0e8d30bb7d8b477-440x550.jpg)
Immunofluorescence analysis of RAW264.7 cells using S100A4 antibody (144-01631) at dilution of 1:100. Blue: DAPI for nuclear staining.![Immunofluorescence - [KO Validated] S100A4 Polyclonal Antibody (144-01631)](https://www.raybiotech.com/files/images/Antibodies/144/144-01631/10.jpg)
Expiration:
12 months from the date of shipment when stored properly.