SAMHD1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9Y3Z3 |
| Gene Id | 25939 |
| Gene Symbols | SAMHD1 |
| Protein Name / Synonyms | SAMHD1, CHBL2, DCIP, HDDC1, MOP-5, SBBI88, deoxynucleoside triphosphate triphosphohydrolase SAMHD1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene may play a role in regulation of the innate immune response. The encoded protein is upregulated in response to viral infection and may be involved in mediation of tumor necrosis factor-alpha proinflammatory responses. Mutations in this gene have been associated with Aicardi-Goutieres syndrome.
Dilutions
WB 1:500 - 1:2000
IF 1:50 - 1:100
IF 1:50 - 1:100
Format
Calculated MW
63kDa/68kDa/72kDa
Observed MW
72kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 387-626 of human SAMHD1 (NP_056289.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SAMHD1 antibody (144-07794) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.![Western blot - [KO Validated] SAMHD1 Polyclonal Antibody (144-07794)](https://cdn.sanity.io/images/g51potcz/production/ce296daafe03334ae98840e4dd932e8439f8d2d4-517x350.jpg)
Western blot analysis of extracts from normal (control) and SAMHD1 knockout (KO) HeLa cells, using SAMHD1 antibody (144-07794) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3min.![Western blot - [KO Validated] SAMHD1 Polyclonal Antibody (144-07794)](https://cdn.sanity.io/images/g51potcz/production/762968a8c9979f7507834146f68e27ec26421561-368x550.jpg)
Immunofluorescence analysis of A549 cells using SAMHD1 antibody (144-07794). Blue: DAPI for nuclear staining.![Immunofluorescence - [KO Validated] SAMHD1 Polyclonal Antibody (144-07794)](https://www.raybiotech.com/files/images/Antibodies/144/144-07794/3.jpg)
Expiration:
12 months from the date of shipment when stored properly.