SCYL1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q96KG9 |
| Gene Id | 57410 |
| Gene Symbols | SCYL1 |
| Protein Name / Synonyms | SCYL1, GKLP, HT019, NKTL, NTKL, P105, SCAR21, TAPK, TEIF, TRAP, SCY1 like pseudokinase 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a transcriptional regulator belonging to the SCY1-like family of kinase-like proteins. The protein has a divergent N-terminal kinase domain that is thought to be catalytically inactive, and can bind specific DNA sequences through its C-terminal domain. It activates transcription of the telomerase reverse transcriptase and DNA polymerase beta genes. The protein has been localized to the nucleus, and also to the cytoplasm and centrosomes during mitosis. Multiple transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:100
Format
Calculated MW
69kDa/78kDa/86kDa/88kDa/89kDa
Observed MW
110kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 460-740 of human SCYL1 (NP_065731.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SCYL1 Antibody (144-06735) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human liver injury using SCYL1 antibody (144-06735) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using SCYL1 antibody (144-06735) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using SCYL1 antibody (144-06735). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.