SERPINB6 antibody [N2C2], Internal, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Bovine (cow) |
| Host Species | Rabbit |
| Gene Id | 5269 |
| Protein Name / Synonyms | SERPINB6, Internal |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Immunohistochemistry, Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
CAP antibody, DFNB91 antibody, DKFZp686I04222 antibody, MGC111370 antibody, MSTP057 antibody, PI6 antibody, PTI antibody, SPI3 antibody, SERPINB6 antibody, "serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 6 antibody", PI-6 antibody, cytoplasmic antiproteinase antibody, serpin B6 antibody, protease inhibitor 6 (placental thrombin inhibitor) antibody, "serpin peptidase inhibitor, clade B (ovalbumin), member 6 antibody"
Specificity
Species Reactivity
Human, Cow
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
43 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 66 and 313 of SERPINB6 (Uniprot ID# P35237)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Immunohistochemistry,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
SERPINB6 antibody [N2C2], Internal detects SERPINB6 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SERPINB6 protein stained by SERPINB6 antibody [N2C2], Internal (107-11135) diluted at 1:500.
Blue: Hoechst 33342 staining.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SERPINB6 protein stained by SERPINB6 antibody [N2C2], Internal (107-11135) diluted at 1:500.
Blue: Hoechst 33342 staining.
Immunohistochemical analysis of paraffin-embedded breast ca, using SERPINB6 antibody(107-11135) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: A549
B: Hela
10% SDS PAGE
107-11135 diluted at 1:1000
A: A549
B: Hela
10% SDS PAGE
107-11135 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.