SGTA Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | O43765 |
| Gene Id | 6449 |
| Gene Symbols | SGTA |
| Protein Name / Synonyms | SGTA, SGT, alphaSGT, hSGT, small glutamine rich tetratricopeptide repeat containing alpha |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a protein which is capable of interacting with the major nonstructural protein of parvovirus H-1 and 70-kDa heat shock cognate protein; however, its function is not known. Since this transcript is expressed ubiquitously in various tissues, this protein may serve a housekeeping function.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:100
Format
Calculated MW
34kDa
Observed MW
38kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human SGTA (NP_003012.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SGTA antibody (144-07306) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.![Western blot - [KO Validated] SGTA Polyclonal Antibody (144-07306)](https://cdn.sanity.io/images/g51potcz/production/30184b9392187c98e08042f864647490833b68c2-403x350.jpg)
Western blot analysis of extracts from normal (control) and SGTA knockout (KO) 293T cells, using SGTA antibody (144-07306) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.![Western blot - [KO Validated] SGTA Polyclonal Antibody (144-07306)](https://cdn.sanity.io/images/g51potcz/production/7b909d288e859ac9101fe5c315299f3818fe714e-350x350.jpg)
Immunohistochemistry of paraffin-embedded human liver cancer using SGTA antibody (144-07306) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] SGTA Polyclonal Antibody (144-07306)](https://cdn.sanity.io/images/g51potcz/production/3553874980b32ecd15518577912e1c3f4654df4f-368x550.jpg)
Immunofluorescence analysis of A-549 cells using SGTA antibody (144-07306). Blue: DAPI for nuclear staining.![Immunofluorescence - [KO Validated] SGTA Polyclonal Antibody (144-07306)](https://www.raybiotech.com/files/images/Antibodies/144/144-07306/4.jpg)
Expiration:
12 months from the date of shipment when stored properly.