SIRT3 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9NTG7 |
| Gene Id | 23410 |
| Gene Symbols | SIRT3 |
| Protein Name / Synonyms | SIRT3, SIR2L3, sirtuin 3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene.
Dilutions
WB 1:500 - 1:1000
IHC 1:20 - 1:50
IHC 1:20 - 1:50
Format
Calculated MW
28kDa/43kDa
Observed MW
27kDa, 44kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human SIRT3 (NP_036371.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SIRT3 antibody (144-05718) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.![Western blot - [KO Validated] SIRT3 Polyclonal Antibody (144-05718)](https://cdn.sanity.io/images/g51potcz/production/58e80df8a33960e53a81577a3650d18c5bd76346-264x350.jpg)
Western blot analysis of extracts from normal (control) and SIRT3 knockout (KO) HeLa cells, using SIRT3 antibody (144-05718) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3min.![Western blot - [KO Validated] SIRT3 Polyclonal Antibody (144-05718)](https://cdn.sanity.io/images/g51potcz/production/56f9bc691bda4053683053e14e9f17bdb57d7955-350x350.jpg)
Immunohistochemistry of paraffin-embedded rat kidney using SIRT3 antibody (144-05718) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] SIRT3 Polyclonal Antibody (144-05718)](https://cdn.sanity.io/images/g51potcz/production/918dfb31f292988db6f8401b33e86ab8eb6cd59b-350x350.jpg)
Immunohistochemistry of paraffin-embedded human liver cancer using SIRT3 antibody (144-05718) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] SIRT3 Polyclonal Antibody (144-05718)](https://cdn.sanity.io/images/g51potcz/production/012ddb6fd22d7ada1993331eced09b021fbf4ac8-350x350.jpg)
Immunohistochemistry of paraffin-embedded human gastric cancer using SIRT3 antibody (144-05718) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] SIRT3 Polyclonal Antibody (144-05718)](https://cdn.sanity.io/images/g51potcz/production/4f4d1df4ae6a40a15d766d5b0037b57deb593033-350x350.jpg)
Immunohistochemistry of paraffin-embedded mouse brain using SIRT3 antibody (144-05718) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] SIRT3 Polyclonal Antibody (144-05718)](https://www.raybiotech.com/files/images/Antibodies/144/144-05718/6.jpg)
Expiration:
12 months from the date of shipment when stored properly.