SMARCA4 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P51532 |
| Gene Id | 6597 |
| Gene Symbols | SMARCA4 |
| Protein Name / Synonyms | SMARCA4, BAF190, BAF190A, BRG1, CSS4, MRD16, RTPS2, SNF2, SNF2L4, SNF2LB, SWI2, hSNF2b, transcription activator BRG1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:20 - 1:50
IHC 1:50 - 1:100
IF 1:20 - 1:50
Format
Calculated MW
181kDa/184kDa
Observed MW
180kDa
Immunogen
A synthetic peptide of human SMARCA4
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SMARCA4 antibody (144-00887) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.![Western blot - [KO Validated] SMARCA4 Polyclonal Antibody (144-00887)](https://cdn.sanity.io/images/g51potcz/production/8cc1d6f553df0f964b2753e43fd9b1d883357082-241x350.jpg)
Western blot analysis of extracts from normal (control) and SMARCA4 knockout (KO) 293T cells, using SMARCA4 antibody (144-00887) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.![Western blot - [KO Validated] SMARCA4 Polyclonal Antibody (144-00887)](https://cdn.sanity.io/images/g51potcz/production/f2912d04faf97fa7f64492b592c85692ec5537d9-350x350.jpg)
Immunohistochemistry of paraffin-embedded human colon using SMARCA4 antibody (144-00887) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] SMARCA4 Polyclonal Antibody (144-00887)](https://cdn.sanity.io/images/g51potcz/production/fe31c470ac28eb09672406b6b6fd44e6c17376c7-350x350.jpg)
Immunohistochemistry of paraffin-embedded human colon carcinoma using SMARCA4 antibody (144-00887) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] SMARCA4 Polyclonal Antibody (144-00887)](https://cdn.sanity.io/images/g51potcz/production/c0acd2d5d23fd4baeacbd5b28a675818fdd99392-468x350.jpg)
Immunofluorescence analysis of HeLa cells using SMARCA4 antibody (144-00887).![Immunofluorescence - [KO Validated] SMARCA4 Polyclonal Antibody (144-00887)](https://www.raybiotech.com/files/images/Antibodies/144/144-00887/5.jpg)
Expiration:
12 months from the date of shipment when stored properly.