SNAI1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O95863 |
| Gene Id | 6615 |
| Gene Symbols | SNAI1 |
| Protein Name / Synonyms | SNAI1, SLUGH2, SNA, SNAH, SNAIL, SNAIL1, dJ710H13.1, zinc finger protein SNAI1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
29kDa
Observed MW
34kDa, 36kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human SNAI1 (NP_005976.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SNAI1 antibody (144-011794) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat kidney using SNAI1 antibody (144-011794) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using SNAI1 antibody (144-011794) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using SNAI1 antibody (144-011794) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.