SNRPA Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P09012 |
| Gene Id | 6626 |
| Gene Symbols | SNRPA |
| Protein Name / Synonyms | SNRPA, Mud1, U1-A, U1A, U1 small nuclear ribonucleoprotein A |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene associates with stem loop II of the U1 small nuclear ribonucleoprotein, which binds the 5' splice site of precursor mRNAs and is required for splicing. The encoded protein autoregulates itself by polyadenylation inhibition of its own pre-mRNA via dimerization and has been implicated in the coupling of splicing and polyadenylation.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
31kDa
Observed MW
33kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-282 of human SNRPA (NP_004587.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SNRPA antibody (144-06410) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human adenomyosis using SNRPA Antibody (144-06410) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using SNRPA Antibody (144-06410) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using SNRPA Antibody (144-06410) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using SNRPA Antibody (144-06410) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using SNRPA Antibody (144-06410) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using SNRPA Antibody (144-06410) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using SNRPA Antibody (144-06410) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using SNRPA Antibody (144-06410) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using SNRPA Antibody (144-06410) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using SNRPA Antibody (144-06410) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using SNRPA Antibody (144-06410) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using SNRPA Antibody (144-06410) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human adenomyosis using SNRPA Antibody (144-06410) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using SNRPA Antibody (144-06410) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using SNRPA Antibody (144-06410) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using SNRPA antibody (144-06410).
Expiration:
12 months from the date of shipment when stored properly.