SNRPE Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P62304 |
| Gene Id | 6635 |
| Gene Symbols | SNRPE |
| Protein Name / Synonyms | SNRPE, HYPT11, SME, Sm-E, snRNP-E, small nuclear ribonucleoprotein E |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is a core component of U small nuclear ribonucleoproteins, which are key components of the pre-mRNA processing spliceosome. The encoded protein plays a role in the 3' end processing of histone transcripts. This protein is one of the targets in the autoimmune disease systemic lupus erythematosus, and mutations in this gene have been associated with hypotrichosis. Several pseudogenes of this gene have been identified.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
10kDa
Observed MW
11kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-92 of human SNRPE (NP_003085.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SNRPE antibody (144-05488) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human kidney using SNRPE Antibody (144-05488) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using SNRPE Antibody (144-05488) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using SNRPE Antibody (144-05488) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using SNRPE Antibody (144-05488) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using SNRPE Antibody (144-05488) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using SNRPE Antibody (144-05488) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using SNRPE Antibody (144-05488) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using SNRPE Antibody (144-05488) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using SNRPE antibody (144-05488). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.