SQSTM1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q13501 |
| Gene Id | 8878 |
| Gene Symbols | SQSTM1 |
| Protein Name / Synonyms | SQSTM1, A170, DMRV, FTDALS3, NADGP, OSIL, PDB3, ZIP3, p60, p62, p62B, sequestosome-1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Research Area | Apoptosis, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
IP 1:20 - 1:50
IHC 1:50 - 1:100
IF 1:50 - 1:100
IP 1:20 - 1:50
Format
Calculated MW
38kDa/47kDa
Observed MW
62kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-440 of human SQSTM1 (NP_001135770.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SQSTM1 antibody (144-011483) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot analysis of extracts from normal (control) and SQSTM1 knockout (KO) 293T cells, using SQSTM1 antibody (144-011483) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human esophagus using SQSTM1 antibody (144-011483) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using SQSTM1 antibody (144-011483) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 100ug extracts of HepG2 cells using 3ug SQSTM1 antibody (144-011483). Western blot was performed from the immunoprecipitate using SQSTM1 antibody (144-011483) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.