The protein encoded by this gene is a type II membrane protein that is thought to catalyze the transfer of sialic acid from CMP-sialic acid to N-linked oligosaccharides and glycoproteins. The encoded protein may be found in the Golgi apparatus and may be involved in the production of polysialic acid, a modulator of the adhesive properties of neural cell adhesion molecule (NCAM1). This protein is a member of glycosyltransferase family 29.
WB 1:500 - 1:2000
IHC 1:50 - 1:200
Recombinant fusion protein containing a sequence corresponding to amino acids 24-160 of human ST8SIA2 (NP_006002.1).
Western blot analysis of extracts of various cell lines, using ST8SIA2 antibody (144-07748) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human stomach using ST8SIA2 antibody (144-07748) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using ST8SIA2 antibody (144-07748) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using ST8SIA2 antibody (144-07748) at dilution of 1:100 (40x lens).
12 months from the date of shipment when stored properly.