Rabbit anti-Stat5 (pTyr694) Antibody
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Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Specificity | The antibody is able to bind to phosphorylated peptide, but not regular peptide in ELISA. It also specifically detects phosphorylated cellular protein derived from stimulated cells. It recognizes Stat5 phosphorylated at Tyr694 in both ELISA and WB. This antibody shows no cross-reactivity with other unrelated protein. |
| Accession Number | NM_003152 |
| Immunogen | Immunogen was synthetic phosphorylated peptide derived from human Stat5. This antibody was obtained from a rabbit immunized with the immunogen. The IgG fraction of immunized serum was purified by two step affinity chromatographies. |
| Immunogen Sequence | CAVDGY(P)VKPQIK |
| Recommended Applications | ELISA (recommended work dilution= 1: 2, 000-4, 000), Western Blotting (recommended work dilution= 2-5 µg/ml), Note: Other applications are not tested yet. Optimal dilutions should be determined by each laboratory for each application. |
| Reconstitution | Supplied as 100 µl of purified antibody in sterile PBS (pH 7.5), containing 0.1% BSA. The final concentration is indicated on the shipping vial. Please avoid freeze-thaw cycles. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Typical Data
Storage/Stability
Always prepare single-use aliquots planning use while receiving package. A small amount of antibody can be kept at 4°C for daily use. For a long storage, recommend to keep them at -20°C after adding an equal volume of glycerol in PBS. It will not lose activity over a year. Minimize freezing and thawing when use.
Expiration
12 months from the date of shipment when stored properly.
References
- Martens N., Uzan G., Wery M., Hooghe R., Hooghe-Peters E.L., Gertler A. Suppressor of cytokine signaling 7 inhibits prolactin, growth hormone, and leptin signaling by interacting with STAT5 or STAT3 and attenuating their nuclear translocation. J. Biol. Chem. 280:13817-13823(2005) [PubMed: 15677474]
- Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell 127:635-648(2006) [PubMed: 17081983]
