Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene encodes a protein that transfers a sulfo moiety to and from estrone, which may control levels of estrogen receptors.
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Recombinant fusion protein containing a sequence corresponding to amino acids 140-294 of human SULT1E1 (NP_005411.1).
Western blot analysis of extracts of various cell lines, using SULT1E1 antibody (144-07452) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunofluorescence analysis of A549 cells using SULT1E1 antibody (144-07452). Blue: DAPI for nuclear staining.
12 months from the date of shipment when stored properly.