TARDBP Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q13148 |
| Gene Id | 23435 |
| Gene Symbols | TARDBP |
| Protein Name / Synonyms | TARDBP, ALS10, TDP-43, TAR DNA binding protein |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP, RIP |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:20 - 1:100
IP 1:50 - 1:100
RIP 1:20 - 1:50
IHC 1:50 - 1:100
IF 1:20 - 1:100
IP 1:50 - 1:100
RIP 1:20 - 1:50
Format
Calculated MW
31kDa/44kDa
Observed MW
42kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human TARDBP (NP_031401.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using TARDBP antibody (144-01183) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts of various cells, using TARDBP antibody (144-01183) at 1:1000 dilution ratio through one-step method.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:4000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human esophagus using TARDBP Antibody (144-01183) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human endometrium using TARDBP Antibody (144-01183) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using TARDBP antibody (144-01183). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.