Rabbit Anti-TBX6 (Center) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | O95947 |
| Gene Id | 6911 |
| Gene Symbols | TBX6 |
| Protein Name / Synonyms | TBX6 (Center) |
| Clonality | Polyclonal |
| Preparation | This TBX6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264-293 amino acids from the Central region of human TBX6. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. Knockout studies in mice indicate that this gene is important for specification of paraxial mesoderm structures.
Specificity
Predicted Reactivity
Rat, Bovine
Other Names
TBX6; T-box transcription factor TBX6
NCBI Accession #
NP_004599.2
Other Accession #
D3ZJK7; P70327; E1BEA8; NP_004599.2
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
47045
Recommended Dilutions
WB: 1:1000; IF: 1:10-50
 | TBX6 Antibody (Center) (Cat# 102-11379) western blot analysis in ZR-75-1 cell line and mouse spleen tissue lysates (35ug/lane).This demonstrates the TBX6 antibody detected the TBX6 protein (arrow). |
 | Fluorescent confocal image of Hela cell stained with TBX6 Antibody (Center)(Cat# 102-11379). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TBX6 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). TBX6 immunoreactivity is localized to nucleus significantly. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.