TCEB2 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q15370 |
| Gene Id | 6923 |
| Gene Symbols | TCEB2 |
| Protein Name / Synonyms | ELOB, SIII, TCEB2, elongin-B |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes the protein elongin B, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. Pseudogenes have been identified on chromosomes 11 and 13.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:20 - 1:50
IHC 1:50 - 1:200
IP 1:20 - 1:50
Format
Calculated MW
13kDa/17kDa
Observed MW
16kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-118 of human TCEB2 (NP_009039.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using TCEB2 antibody (144-60305) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human colon using TCEB2 antibody (144-60305) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using TCEB2 antibody (144-60305) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using TCEB2 antibody (144-60305) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 150ug extracts of MCF7 cells using 3ug TCEB2 antibody (144-60305). Western blot was performed from the immunoprecipitate using TCEB2 antibody (144-60305) at a dilition of 1:500.
Expiration:
12 months from the date of shipment when stored properly.