TCP1 theta antibody [N2C2], Internal, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 10694 |
| Protein Name / Synonyms | TCP1 theta, Internal |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
C21orf112 antibody, Cctq antibody, D21S246 antibody, KIAA0002 antibody, PRED71 antibody, CCT8 antibody, T-complex protein 1 subunit theta antibody, TCP-1-theta antibody, renal carcinoma antigen NY-REN-15 antibody, "T-complex protein 1, theta subunit antibody", CCT-theta antibody, "chaperonin containing TCP1, subunit 8 (theta) antibody"
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
60 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 111 and 367 of TCP1 theta (Uniprot ID# P50990)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
107-10555 diluted at 1:5000
A: Mouse brain
7.5% SDS PAGE
107-10555 diluted at 1:5000
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using TCP-1 theta (107-10555) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: A431
B: H1299
C: Hela
7.5% SDS PAGE
107-10555 diluted at 1:1000
A: A431
B: H1299
C: Hela
7.5% SDS PAGE
107-10555 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.