TJP2 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q9UDY2 |
| Gene Id | 9414 |
| Gene Symbols | TJP2 |
| Protein Name / Synonyms | TJP2, C9DUPq21.11, DFNA51, DUP9q21.11, PFIC4, X104, ZO2, tight junction protein 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a zonula occluden that is a member of the membrane-associated guanylate kinase homolog family. The encoded protein functions as a component of the tight junction barrier in epithelial and endothelial cells and is necessary for proper assembly of tight junctions. Mutations in this gene have been identified in patients with hypercholanemia, and genomic duplication of a 270 kb region including this gene causes autosomal dominant deafness-51. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
111-137kDa
Observed MW
160kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 951-1190 of human TJP2 (NP_004808.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using TJP2 antibody (144-00594) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat spleen using TJP2 antibody (144-00594) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using TJP2 antibody (144-00594) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using TJP2 antibody (144-00594) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using TJP2 antibody (144-00594) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using TJP2 antibody (144-00594) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using TJP2 antibody (144-00594) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.