TMPO Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P42166|P42167 |
| Gene Id | 7112 |
| Gene Symbols | TMPO |
| Protein Name / Synonyms | TMPO, CMD1T, LAP2, LEMD4, PRO0868, TP, thymopoietin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene resides in the nucleus and may play a role in the assembly of the nuclear lamina, and thus help maintain the structural organization of the nuclear envelope. It may function as a receptor for the attachment of lamin filaments to the inner nuclear membrane. Mutations in this gene are associated with dilated cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
38kDa/50kDa/75kDa
Observed MW
56kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-410 of human TMPO (NP_001027454.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using TMPO antibody (144-02534) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.![Western blot - [KO Validated] TMPO Polyclonal Antibody (144-02534)](https://cdn.sanity.io/images/g51potcz/production/af9b777178c40a25227c1363ff70fdf7d090a8ad-453x350.jpg)
Western blot analysis of extracts from normal (control) and TMPO knockout (KO) 293T cells, using TMPO antibody (144-02534) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.![Western blot - [KO Validated] TMPO Polyclonal Antibody (144-02534)](https://cdn.sanity.io/images/g51potcz/production/538bfa565199540c50f3386923908c77d73ca0ad-467x350.jpg)
Immunohistochemistry of paraffin-embedded rat brain using TMPO Antibody (144-02534) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] TMPO Polyclonal Antibody (144-02534)](https://cdn.sanity.io/images/g51potcz/production/0265bcf2c8169a76dbc4b9c44f696f55b518de25-467x350.jpg)
Immunohistochemistry of paraffin-embedded human tonsil using TMPO Antibody (144-02534) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] TMPO Polyclonal Antibody (144-02534)](https://cdn.sanity.io/images/g51potcz/production/2b7e3e72a2bc7b956e3665f31ef929ac921fed38-467x350.jpg)
Immunohistochemistry of paraffin-embedded mouse brain using TMPO Antibody (144-02534) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] TMPO Polyclonal Antibody (144-02534)](https://cdn.sanity.io/images/g51potcz/production/556a406e5c4a10cbc3a6c15fe81c4bd7fb8f26e6-468x350.jpg)
Immunofluorescence analysis of U2OS cells using TMPO antibody (144-02534).![Immunofluorescence - [KO Validated] TMPO Polyclonal Antibody (144-02534)](https://www.raybiotech.com/files/images/Antibodies/144/144-02534/6.jpg)
Expiration:
12 months from the date of shipment when stored properly.