Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q15388
Gene Id	9804
Gene Symbols	TOMM20
Protein Name / Synonyms	TOMM20, MAS20, MOM19, TOM20, translocase of outer mitochondrial membrane 20
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:20 - 1:50

Format

Calculated MW

16kDa

Observed MW

16kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-145 of human TOMM20 (NP_055580.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using TOMM20 antibody (144-06774) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Western blot - TOMM20 antibody (144-06774)

Immunohistochemistry of paraffin-embedded human liver cancer using TOMM20 antibody (144-06774) at dilution of 1:100 (40x lens).
Immunohistochemistry - TOMM20 antibody (144-06774)

Immunohistochemistry of paraffin-embedded human colon carcinoma using TOMM20 antibody (144-06774) at dilution of 1:100 (40x lens).
Immunohistochemistry - TOMM20 antibody (144-06774)

Immunohistochemistry of paraffin-embedded human esophageal cancer using TOMM20 antibody (144-06774) at dilution of 1:100 (40x lens).
Immunohistochemistry - TOMM20 antibody (144-06774)

Immunohistochemistry of paraffin-embedded human gastric cancer using TOMM20 antibody (144-06774) at dilution of 1:100 (40x lens).
Immunohistochemistry - TOMM20 antibody (144-06774)

Immunohistochemistry of paraffin-embedded mouse kidney using TOMM20 antibody (144-06774) at dilution of 1:100 (40x lens).
Immunohistochemistry - TOMM20 antibody (144-06774)

Immunohistochemistry of paraffin-embedded mouse heart using TOMM20 antibody (144-06774) at dilution of 1:100 (40x lens).
Immunohistochemistry - TOMM20 antibody (144-06774)

Immunofluorescence analysis of A549 cells using TOMM20 antibody (144-06774). Blue: DAPI for nuclear staining.
Immunofluorescence - TOMM20 antibody (144-06774)

Immunofluorescence analysis of HeLa cells using TOMM20 antibody (144-06774) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence - TOMM20 antibody (144-06774)

Expiration:

12 months from the date of shipment when stored properly.