This gene encodes a transcription factor that represses GATA-regulated genes and binds to a dynein light chain protein. Binding of the encoded protein to the dynein light chain protein affects binding to GATA consensus sequences and suppresses its transcriptional activity. Defects in this gene are a cause of tricho-rhino-phalangeal syndrome (TRPS) types I-III.
Dilutions
WB 1:500 - 1:2000 IF 1:50 - 1:100
Format
Calculated MW
141kDa/143kDa
Observed MW
120kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 20-260 of human TRPS1 (NP_054831.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using TRPS1 antibody (144-07743) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunofluorescence analysis of A549 cells using TRPS1 antibody (144-07743).
Expiration:
12 months from the date of shipment when stored properly.
