TSHR Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P16473 |
| Gene Id | 7253 |
| Gene Symbols | TSHR |
| Protein Name / Synonyms | TSHR, CHNG1, LGR3, hTSHR-I, thyrotropin receptor |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Cancer, Autoimmune Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is a membrane protein and a major controller of thyroid cell metabolism. The encoded protein is a receptor for thyrothropin and thyrostimulin, and its activity is mediated by adenylate cyclase. Defects in this gene are a cause of several types of hyperthyroidism. Three transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:20- 1:50
IHC 1:50 - 1:200
IF 1:20- 1:50
Format
Calculated MW
28kDa/30kDa/86kDa
Observed MW
110kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 21-253 of human TSHR (NP_001018046.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using TSHR antibody (144-06781) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using TSHR Antibody (144-06781) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using TSHR Antibody (144-06781) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using TSHR Antibody (144-06781) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.