The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:20 - 1:50
Format
Calculated MW
9kDa/11kDa
Observed MW
12kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 20 to the C-terminus of human TXN (NP_003320.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using TXN antibody (144-07638) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse lung using TXN antibody (144-07638) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.
