TYMS Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P04818 |
| Gene Id | 7298 |
| Gene Symbols | TYMS |
| Protein Name / Synonyms | TYMS, HST422, TMS, TS, thymidylate synthase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase.
Dilutions
WB 1:500 - 1:2000
IF 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
26kDa/31kDa/35kDa
Observed MW
36kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-313 of human TYMS (NP_001062.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using TYMS antibody (144-010441) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.
Immunofluorescence analysis of C6 cells using TYMS antibody (144-010441) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using TYMS antibody (144-010441) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using TYMS antibody (144-010441) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.