UBE2O Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9C0C9 |
| Gene Id | 63893 |
| Gene Symbols | UBE2O |
| Protein Name / Synonyms | UBE2O, E2-230K, ubiquitin conjugating enzyme E2 O |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Dilutions
WB 1:1000 - 1:4000
IP 1:50 - 1:200
IP 1:50 - 1:200
Format
Calculated MW
141kDa
Observed MW
200kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1073-1292 of human UBE2O (NP_071349.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using UBE2O antibody (144-10036) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.![Western blot - [KO Validated] UBE2O Polyclonal Antibody (144-10036)](https://cdn.sanity.io/images/g51potcz/production/6f796ef3be52e26b2a5662e992cdcd6c927d02b3-479x350.jpg)
Western blot analysis of extracts from normal (control) and UBE2O knockout (KO) HeLa cells, using UBE2O antibody (144-10036) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.![Western blot - [KO Validated] UBE2O Polyclonal Antibody (144-10036)](https://www.raybiotech.com/files/images/Antibodies/144/144-10036/2.jpg)
Immunoprecipitation analysis of 200ug extracts of A-431 cells using 3ug UBE2O antibody (144-10036). Western blot was performed from the immunoprecipitate using UBE2O antibody (144-10036) at a dilition of 1:1000.![Immunoprecipitation - [KO Validated] UBE2O Polyclonal Antibody (144-10036)](https://www.raybiotech.com/files/images/Antibodies/144/144-10036/3.jpg)
Expiration:
12 months from the date of shipment when stored properly.