UBE2R2 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q712K3 |
| Gene Id | 54926 |
| Gene Symbols | UBE2R2 |
| Protein Name / Synonyms | UBE2R2, CDC34B, E2-CDC34B, UBC3B, ubiquitin-conjugating enzyme E2 R2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
Protein kinase CK2 is a ubiquitous and pleiotropic Ser/Thr protein kinase involved in cell growth and transformation. This gene encodes a protein similar to the E2 ubiquitin conjugating enzyme UBC3/CDC34. Studies suggest that CK2-dependent phosphorylation of this ubiquitin-conjugating enzyme functions by regulating beta-TrCP substrate recognition and induces its interaction with beta-TrCP, enhancing beta-catenin degradation.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
27kDa
Observed MW
27kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-238 of human UBE2R2 (NP_060281.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using UBE2R2 antibody (144-07373) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded mouse cancer using UBE2R2 antibody (144-07373) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using UBE2R2 antibody (144-07373).
Expiration:
12 months from the date of shipment when stored properly.