Protein kinase CK2 is a ubiquitous and pleiotropic Ser/Thr protein kinase involved in cell growth and transformation. This gene encodes a protein similar to the E2 ubiquitin conjugating enzyme UBC3/CDC34. Studies suggest that CK2-dependent phosphorylation of this ubiquitin-conjugating enzyme functions by regulating beta-TrCP substrate recognition and induces its interaction with beta-TrCP, enhancing beta-catenin degradation.
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Recombinant fusion protein containing a sequence corresponding to amino acids 1-238 of human UBE2R2 (NP_060281.2).
Western blot analysis of extracts of various cell lines, using UBE2R2 antibody (144-07373) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded mouse cancer using UBE2R2 antibody (144-07373) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using UBE2R2 antibody (144-07373).
12 months from the date of shipment when stored properly.