UBE2S Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q16763 |
| Gene Id | 27338 |
| Gene Symbols | UBE2S |
| Protein Name / Synonyms | UBE2S, E2-EPF, E2EPF, EPF5, ubiquitin-conjugating enzyme E2 S |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins.
Dilutions
WB 1:200 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
23kDa
Observed MW
24kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 122-221 of human UBE2S (NP_055316.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using UBE2S antibody (144-04658) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human colon carcinoma using UBE2S antibody (144-04658) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate cancer using UBE2S antibody (144-04658) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using UBE2S antibody (144-04658) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.