UBE2Z Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q9H832 |
| Gene Id | 65264 |
| Gene Symbols | UBE2Z |
| Protein Name / Synonyms | UBE2Z, HOYS7, USE1, ubiquitin-conjugating enzyme E2 Z |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes an enzyme which ubiquitinates proteins which participate in signaling pathways and apoptosis.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:100
Format
Calculated MW
28kDa/38kDa
Observed MW
25kDa, 39kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 115-354 of human UBE2Z (NP_075567.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using UBE2Z antibody (144-07225) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human kidney using UBE2Z antibody (144-07225) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using UBE2Z antibody (144-07225) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A-549 cells using UBE2Z antibody (144-07225). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.