WB 1:500 - 1:2000
IF 1:50 - 1:100
Recombinant fusion protein containing a sequence corresponding to amino acids 1-328 of human UCHL5 (NP_001186190.1).
Western blot analysis of extracts of various cell lines, using UCHL5 antibody (144-07978) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 40s.
Immunofluorescence analysis of A549 cells using UCHL5 antibody (144-07978). Blue: DAPI for nuclear staining.
12 months from the date of shipment when stored properly.